Osthole is main active ingredients of Cnidifolia extract include pinene, camphene, bornyl is ovalarate, is oborneol, osthole, cindimine, cnidiadin, isopimpinelline, etc.
【Molecular formula】 C15H16O3
【Molecular Weight】 244.29
[CAS No.] 484-12-8
【Source】 Cnidium monnieric (L.) Cuss, an annual herbaceous plant of Umbelliferae. fruit.
【Specification】 10%, 20%, 35%, 50%, 90%, 95%, 98%
【Origin】Sichuan, Zhejiang
Introduction:
Osthole is a substance extracted from the fruit of the annual herbaceous plant Cnidium chinensis in the Umbelliferae family. Its appearance is yellow-green to white crystalline powder, insoluble in water and cold petroleum ether, and easily soluble in acetone, methanol, ethanol, and chloroform , chloroform, ethyl acetate, soluble in boiling petroleum ether, has antispasmodic, lowering blood pressure, antiarrhythmic, enhancing immune function and broad-spectrum antibacterial effects. Clinically, it is mainly used to treat male impotence, scrotum itching, female vaginal itching, cold uterus infertility, rheumatic arthralgia, scabies and eczema, and trichomonal vaginitis. Osthole can also be used as a field insecticide and fungicide, mainly with contact action and supplemented by stomach poisoning. The medicinal liquid enters the insect body through body surface absorption, acts on the nervous system of the pest, and causes non-functional muscle contraction of the pest. It eventually dies of exhaustion, and has good contact killing effect on various pests such as cabbage caterpillar, tea looper, cotton bollworm, beet armyworm and various aphids.
physical properties
Conventional 35%, 50% and other low content is yellow-green powder, high content is white needle-shaped crystal powder. Soluble in alkali solution, methanol, ethanol, chloroform, acetone, ethyl acetate and boiling petroleum ether, etc., insoluble in water and petroleum ether, mp.83℃～84℃, bp.145℃～150℃.
Pharmacological effect
Osthole has antispasmodic, hypotensive, antiarrhythmic, immune function enhancing and broad-spectrum antibacterial effects. Warming the kidney and strengthening yang, drying dampness, expelling wind, and killing parasites. For impotence, cold uterus, infertility, cold-damp leukorrhagia, wet nose and low back pain; external treatment of genital eczema, vulva itching in women, and trichomonal vaginitis.
QC
① Chromatography (high performance liquid chromatography: thin layer chromatography; gas chromatography)
②Mass spectrometry (liquid-mass spectrometry)
③Spectrum (infrared, ultraviolet), atomic absorption
④ Determination (determination of physical constants; determination of polarimetry; determination of non-volatile matter; determination of loss on drying; Karl Fischer determination; determination of evaporation or ignition residue)
⑤ Analysis (content analysis; elemental analysis)
⑥ Titration (acid titration; alkaline titration; coordination titration; non-aqueous titration; redox titration)
⑦ Test (mixing test with water; clarity test; various chemical reaction tests)
Efficacy:
Osthole has antispasmodic, hypotensive, anti-arrhythmic, immune-enhancing and broad-spectrum antibacterial effects. Osthole not only has anti-hypertension, anti-arrhythmia, anti-inflammation, anti-tumor, anti-osteoporosis and other effects, but also has significant effects on pests and plant pathogens as a new type of biological pesticide.
botanical pesticides
Osthole is a class of natural coumarin compounds firstly extracted and isolated from Umbelliferae. The dry and mature fruit Cnidinia (Fructuscnidii) of Umbelliferae Cnidii is relatively high in content, hence the name. Relevant studies have shown that osthole is also present in many other plants, mainly including Umbelliferae such as Angelica, Gudang, and Cnidium, as well as Rutaceae such as Citrus, Champagne, Citrus, and Rutacea. , In addition, it is also distributed in a few species of Asteraceae and Fabaceae. Osthole has another name called methoxyparscreol or parshole (osthole, Ost), its chemical name is 7-methoxy-8-prenyl coumarin, and its core structure consists of benzene ring and pyrene Composition of the ketone ring. As a member of coumarins, osthole has not only the core structure of coumarin, but also an isopentene structure, and the isopentene structure compound plays an important role in disease resistance as plant protection. Osthole is a plant-derived insecticide Chemicalbook. Its mode of action is mainly contact action, supplemented by stomach poisoning. The liquid is absorbed into the insect body through the body surface and acts on the nervous system of the pest, resulting in non-functional muscle contraction of the pest. Eventually exhausted and died. The indoor activity and toxicity test shows that it has high activity against cabbage caterpillar (LD50 is 6.2258mg/kg). The field efficacy test shows that it has good control effect on cruciferous vegetable cabbage caterpillar and tea tree geometrid. The dosage for preventing and controlling cruciferous vegetable cabbage caterpillar is 4.8~7.2g/h㎡ of active ingredient (converted into 0.4% osthole emulsifiable oil, the commercial volume is 80~120mL per mu); the dosage for controlling tea tree worm is 6~7.2 g/h㎡ (converted into 0.4% osthole emulsifiable oil, use 100~120mL per mu of land). Generally, the amount of drug solution per mu is 50~75kg, and it is sprayed evenly; the duration of effect is about 7 days. It is safe to crops within the dosage range, and no phytotoxicity has been seen. chemical properties
1.3.1 Extraction, separation and purification process
Lovage → sweating → crushing and sieving → ethanol extraction → ethyl acetate extraction → separation and purification of macroporous adsorption resin → calculation of osthole content.
1.3.2 Determination of osthole content
Establish a standard curve according to the method of literature [19], and carry out linear regression with the mass concentration X of osthole as the abscissa and the absorbance Y as the ordinate, and the linear regression equation of osthole is:
Y=61.336 7X+0.259 82R2=0.999 47
1.3.3 Single factor experiment
(1) Investigate the influence of solid-liquid ratio on the yield of osthole
The extraction temperature was fixed at 60 °C, the extraction time was 60 min, and the ethanol concentration was 60%. According to the method in 1.3.1, the ratio of solid to liquid was 1:10, 1:15, 1:20, 1:25, and 1:30. Effect of osthole yield.
(2) Investigate the influence of extraction temperature on the yield of osthole
With a fixed solid-liquid ratio of 1:20, extraction time of 60 min, and ethanol concentration of 60%, the effects of extraction temperatures of 30 ℃, 60 ℃, 75 ℃, and 90 ℃ on the yield of osthole were investigated according to the method of 1.3.1.
(3) Investigate the influence of extraction time on the yield of osthole
With a fixed solid-liquid ratio of 1:20, extraction temperature of 60 °C, and ethanol concentration of 60%, the effects of extraction time of 30 min, 45 min, 60 min, and 90 min on the yield of osthole were investigated according to the method of 1.3.1.
(4) Investigate the influence of ethanol concentration on the yield of osthole
With a fixed solid-liquid ratio of 1:20, extraction time of 60 min, and extraction temperature of 60 °C, the effects of ethanol concentrations of 50%, 60%, 70%, and 90% on the yield of osthole were investigated according to the method of 1.3.1.
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