Dihydroquercetin is usually used as a raw material for high-end foods. It is an extract of the pinaceae plants such as larch, Douglas fir, golden willow tree, and yellow wolfberry leaves. It is also a flavonoid plant compound.
Dihydroquercetin is a natural antioxidant that can be used in cosmetics. Its extraction source - larch is mainly produced in temperate deciduous broad-leaved forests and subarctic evergreen broad-leaved forests. Russia, located north of China, is prolific in larch; and dihydroquercetin is mainly extracted from the roots of larch. It is a natural cosmetic raw material and can also be used as a high-end food raw material.
Background and Overview
Dihydroquerpisol is a dihydroflavonol compound that belongs to the vitamin P family and is a widely used bioactive agent. Like other flavonoids, it has a variety of biological activities in the human body, including antioxidant , free radical scavenging, antiviral, anti-inflammatory, vasodilation and antibacterial effects. Dihydroquercetin is a new food antioxidant in the food industry. Its antioxidant capacity is comparable to many natural and synthetic antioxidants. It has not been found to have toxic reactions when used in large doses, and no experiments have found that it has teratogenic, allergic or mutagenic effects on the fetus.
Preparation
The processes for obtaining dihydroquercetin are mostly divided into three categories: ① Using Huangfei leaves or rose stems as raw materials, first extract the glycoside derivatives of dihydroquercetin, and then obtain dihydroquercetin through acid hydrolysis Dihydroquercetin monomer, the yield of this process literature is generally about 1%; ② Synthetic route; ③ Using larch bark or sawdust as raw material, dihydroquercetin monomer is directly extracted, and a method is provided in patent CN1858046 Using adsorption method to extract dihydroquercetin from larch, the yield of this process is only close to 1%.
The extraction process using Huangfei leaves and other plants as raw materials involves a hydrolysis process, which results in a long experimental cycle, resulting in low production efficiency; the synthesis route is complex and the operation is very difficult.
1. CN201010578835.7 overcomes the deficiencies in the prior art and provides a method for extracting dihydroquercetin from larch sawdust. The technical solution of the present invention is summarized as follows:
(1) Extraction: Crush the raw material larch sawdust, add 7 to 10 mass times of ethanol aqueous solution with a volume percentage of 0% to 30%, and reflux for extraction, and concentrate the extract under reduced pressure to 1/15 to 1/1 of the original volume. 10; Then add an ethanol aqueous solution with a volume concentration of 85% to 98% equivalent to 1 to 3 times the volume of the concentrated solution to precipitate the arabinogalactan in it, filter, and wash the filter residue with an ethanol aqueous solution with a volume concentration of 95%. Combine the filtrate and washing liquid, and concentrate under reduced pressure until there is no alcohol smell;
(2) Purification: The concentrated solution obtained in step (1) is subjected to liquid phase adsorption separation with macroporous resin, and then deionized water, ethanol with a volume concentration of 50%, a volume concentration of 70% and a volume concentration of 95% are used in sequence. Aqueous solution gradient elution, the effluent is collected in sections, concentrated and dried into powder, and the obtained powders are separately subjected to liquid phase detection. The measured dihydroquercetin content is the crude dihydroquercetin;
(3) Crystallization: Dissolve the crude dihydroquercetin in hot water, stir and cool, place it in a freezer for recrystallization to obtain a beige precipitate, and then recrystallize it with methanol to obtain dihydroquercetin.
2. CN201210590429.1 uses rutin as raw material to obtain quercetin through hydrolysis, and then uses quercetin as raw material to prepare dihydroquercetin. At the same time, rhamnose is extracted from the acid water waste liquid of rutin hydrolysis. The raw material Easy to obtain, simple process, green and environmentally friendly, suitable for industrial production.
Pharmacological effects
Dihydroquercetin has a variety of physiological functions.
1) Antioxidant effect: Antioxidant protects capillaries, 3 to 5 times stronger than quercetin, resists lipid peroxidation, protects liver, nerve cells, red blood cells, resists myocardial necrosis, etc.
2) Effect on enzyme activity: Dihydroquercetin can inhibit the esterification of cholesterol and the synthesis of triacylglycerol and phospholipids in liver cancer cells. Dihydroquercetin can inhibit the activity of viral enzymes and induce an increase in intracellular enzyme activity, but does not change the distribution. Inhibits aldehyde reductase. It has a weak inhibitory effect on protein kinases.
3) Effect on cells: It can effectively inhibit leukemia cells and malignant lymphocytes. It is active against mouse leukemia P388, and the life extension rates of 150 and 100 mg/kg are 40% and 37% respectively. It has a mild reducing effect on cytochrome C.
4) Antibacterial effect: It has strong antibacterial effect on Staphylococcus aureus, Escherichia coli, Shigella dysenteriae and Typhoid bacilli.
In the pharmaceutical field, dihydroquercetin's antioxidant and anti-peroxidative abilities make it useful in the treatment of oxidative damage diseases caused by free radicals. It can also be used as an anti-radiation drug for patients undergoing radiotherapy or those engaged in For radioactive workers, dihydroquercetin can also be used as an intermediate in the synthesis of other drug molecules, such as the synthesis of a series of silymarin compounds and the drug cyanidin for the treatment of cardiovascular diseases.
In the industrial field, dihydroquercetin has been added to some cosmetics as the main functional ingredient. It can also be added as an oxidation stabilizer to rocket fuels, various hydrocarbon fuels, generator oils and industrial greases. middle.
Preparation
An oral solid dispersion preparation of dihydroquercetin, which consists of: dihydroquercetin solid dispersion and auxiliary materials; wherein, the dihydroquercetin solid dispersion is composed of dihydroquercetin and solid Dispersion carrier composition. Weigh 200g of dihydroquercetin raw material, crush it through a 100 mesh sieve, and dissolve it with 1 liter of absolute ethanol; weigh 1200g of polyvinylpyrrolidone PVP-k30, crush it through a 100 mesh sieve, and add dihydroquercetin absolute ethanol. liquid, stir to dissolve; place the ethanol liquid in a 55°C water bath to rotate and evaporate to recover the solvent to dryness; take it out, balance it in a desiccator for 24 hours, crush it through a 100 mesh sieve, and obtain dihydroquercetin PVP-k30 solid dispersion ; Weigh 300g of lactose and 300g of microcrystalline cellulose, add them to the prepared dihydroquercetin solid dispersion, mix well, add 20g of lubricant magnesium stearate, and directly press the whole powder into tablets to obtain.
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