Introduction
Fucoidan is a unique water-soluble polysaccharide with sulfuric acid groups, also known as fucoidan sulfate fucoidan, which mainly exists in brown algae and some Marine invertebrates. Low molecular weight of fucoidan has anticoagulant, antiviral, antithrombotic, antitumor and immunity enhancement activities. A large number of scientific studies have proved that the fucoidan has more than 20 physiological effects such as regulating immunity, clearing free radicals, anti-aging, anti-coagulation and anti-thrombosis and HIV virus, eliminating gastrointestinal system disorders, anti-allergy, enhancing liver function, reducing hyperlipidemia and hypertension, stabilizing blood sugar levels, and promoting skin regeneration and skin moisturizing.
Function
Regulation of immunity:
Fucoidan has the function of bidirectional regulation of immunity, which can enhance human immunity and remove abnormal cells.
Antioxidant and anti-aging:
Fucoidan have the ability to scavenge free radicals, which helps fight oxidative stress and anti-aging.
Antiviral
It has extensive viral inhibitory effect on influenza virus, human papillomavirus, HIV virus and herpes simplex virus.
Prevention and treatment of Helicobacter pylori infection:
Fucoidan can specifically adhere to the fucoidan-binding protein on Helicobacter pylori, so as to target the elimination of Helicobacter pylori.
Application
Cosmetic field
Inhibit melanin production
Fucoidan can effectively inhibit the production of melanin, through the absorption of ultraviolet light can help to inhibit the activity of tyrosamic acid, can effectively inhibit the production of skin melanin, effectively prevent the appearance of spots, the effect is very good, but also this very good anti-radiation effect.
antioxidant
Fucoidan can also help anti-oxidation, algae polysaccharide has a large number of anthocyanins and vitamins, can help delay aging, and can neutralize the excess free radicals in the human blood, can delay the speed of aging, which is very friendly for female friends.
Whitening and moisturizing
Fucoidan can also help to whiten and moisturize. The composition of fucoidan can help to remove excess free radicals in the human body, and has an inhibitory effect on tyrosinase activity. The effect of whitening and moisturizing is very good, and it can meet the needs of different skin types.
Medical and health care field
The fucoidan of rock algae has strong antioxidant activity, which can effectively remove free radicals and reduce the damage of oxidative stress on human body. Fucoidan plays an anti-tumor role by enhancing immunity and inhibiting tumor growth. In addition, fucoidan can effectively reduce the levels of cholesterol and triacylglycerol, which is of great significance for the prevention of cardiovascular diseases. By activating immune cells and regulating the production of immune factors, enhance the body's immunity and improve the body's resistance.
Food field
The fucoidan can be used as an additive of functional food to increase its nutritional value and improve human health. As a kind of natural antibacterial agent and inhibitor, the fucoidan in food preservation can extend the preservation period of food and reduce the deterioration of food.
Agricultural field
As a plant growth regulator, fucoidan can promote the growth and development of crops and increase the yield. At the same time, it can also enhance the resistance of crops. It can be used as an alternative to pesticides, has a certain inhibitory effect on some agricultural pests, and can be used as an environmental protection material to replace pesticides.
Production Method
1. A fucoidan-rich bacterial extracellular polysaccharide extraction method, which is characterised as follows: a strain of Hirschsprungia XPF-1 is ventilated and cultured to obtain the fucoidan-rich bacterial extracellular polysaccharide fermentation broth: then centrifuged to obtain the supernatant, and the obtained supernatant is concentrated to obtain the cut-off solution by ultrafiltration, and then added to the alcohol precipitation, and then centrifuged to obtain the sediment, and then centrifuged and washed with a small amount of ethanol for 2-4 times, and then dried and crushed to obtain the crude product of fucose-rich bacterial extracellular polysaccharide after low-temperature drying of 60 ℃ or less. 2. Vacuum drying, crushing that is rich in fucoidan bacterial extracellular polysaccharide crude products.2. The extraction method of fucoidan-rich bacterial extracellular polysaccharide described in claim 1, characterised by the specific steps as follows:
(1) Fermentation: using Escherichia helvetica XPF-1 as the starting strain.
The seed medium was composed of glucose 10g/L, yeast 3g/L, wort extract 3g/L, tryptone 5g/L, pH 7.0, prepared with purified water and sterilised at 121℃ for 20 min; agar 20g/L was added to the solid medium.
A single colony of Escherichia coli XPF-1 was picked with an inoculating needle and inoculated into a 500mL triangular shaker containing 100mL of seed medium, and then placed in a shaker to incubate at 250rpm for 18~30 h. The culture was then continued in the fermentation medium.
Then the culture was continued in the fermentation medium, which consisted of maltose 30g/L, compound nitrogen source 5g/L, K.HPO,2g/L, MgSO,-7H20 0.1g/L, and the initial pH 6.0, and was prepared with purified water;
The fermentation temperature was 30°C, the inoculum amount was 5% by volume, the volume of the fermenter was 20L, the filling volume was 14L, the stirring speed was 500~800rpm, and the ventilation intensity was 1. 0vvm :the ventilation fermentation was carried out for 4d to obtain the fucose-rich extracellular polysaccharide fermentation broth:.
(2) First centrifugation: centrifuge the fucoidan-rich extracellular polysaccharide fermentation broth obtained in step (1) at 5000~10000 r/min for 10-30 min, and obtain the supernatant.
(3) ultrafiltration: the supernatant obtained was concentrated by ultrafiltration, the molecular weight cut-off of ultrafiltration membrane was 10,000~40,000 Da, and the ultrafiltration operating pressure was 0.15~0.3 MPa, and the retention solution was obtained: (4) alcohol dialysis and the second centrifugation: alcohol solvent was added into the retention solution obtained from the step (3), and the ratio of the volume of alcohol solvent to the retention solution was 1.5-3 : 1, and the retention solution was allowed to dialyse for 3h, and then centrifuged for 10-30 min under 3000-8000 r/min, to obtain the supernatant: (5) the supernatant obtained from the step (1) was concentrated by ultrafiltration. Centrifugation was performed at 3000-8000 r/min for 10-20 min, and the precipitate was collected;
(5) washing: add a small amount of alcohol solvent to the precipitate obtained in step (4) for washing, and centrifuge again to collect the precipitate: the volume ratio of the alcohol solvent added in the washing process is 0.3-0.8 times the volume of solids, and the centrifugal washing process is 3000-8000 r/min, and centrifugation time is 10-20 min: centrifugal washing is performed 2-4 times; and
(6) Post-treatment: the washed precipitate collected in step (5) is dried and crushed at an operating temperature of not more than 60 ℃, vacuum dried at 0.05-0. 10MPa for 20-26h, and then crushed to 200-400 mesh, to obtain the product fucose-rich bacterial extracellular naming sugar.
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