Dipotassium glycyrrhizinate (DPG for short) is an active ingredient extracted from the roots of the leguminous plant licorice (Glycyrrhizauralensis Fisch.).
Dipotassium glycyrrhizinate can inhibit the production of melanin. The back skin of guinea pigs was selected for the experimental study. Under UVB irradiation, the skin pretreated with 0.5% dipotassium glycyrrhizinate had a higher whiteness coefficient than the skin of the control group, and the effect was significant. The experiment The results show that dipotassium glycyrrhizinate can significantly inhibit melanin production and can be used to prevent skin pigmentation and melanin production after sun exposure. It is a potential skin whitening agent.
Chemical Properties: Off-white to light yellow powder, odorless. It has a special sweetness, about 150 times as sweet as sucrose, and stays sweet for a long time. It is easily soluble in water, soluble in dilute ethanol, glycerol, and propylene glycol, and slightly soluble in absolute ethanol and ether.
Usage: Mainly used in medicine, food, beverage and health care products industries. Dipotassium glycyrrhizinate can be used to improve the dissolution of amphotericin B (AMB) and the bioavailability of the drug in suppositories.
Purpose and application scope: In the pharmaceutical field, dipotassium glycyrrhizinate can be used in eye drops and ointments; in the cosmetics industry, it can be used in sunscreen, freckle removal creams, skin care lotions, lotions, etc.; in the daily chemical industry, it can be used in toothpaste; in the food industry , can be used as potassium supplement, sweetener, preservative, and flavoring in sports drinks.
Production method: Using licorice as raw material, it is extracted with water and then added with potassium hydroxide or potassium carbonate for complete neutralization.
Identify:
(1) Take 0.2g of this product, add 5ml of water, 3ml of hydrochloric acid, and add 2, 4-dinitrophenylhydrazine ethanol test solution 2 ~ 3 drops into the distillation solution to produce orange-red precipitate.
(2) The residue left under the incandescent residue of this product is checked according to law (Appendix III of the second part of the Chinese Pharmacopoeia 2000 edition) for potassium salt identification reaction.
Check: Clarification After taking 1.0g of this product and adding 20ml of water to dissolve, the solution should be clarified.
Acidity: Take 1.0g of this product, add 100ml of water and dissolve it, then determine according to law (Appendix VI H of the second part of the Chinese Pharmacopoeia 2000 edition), the pH value should be 5.0 ~ 6.0.
Chloride: Take 0.5g of this product, check according to law (Appendix VIII A of Part II of the Chinese Pharmacopoeia 2000 Edition), and compare with the control solution made of 7.0ml of standard sodium chloride solution, not more concentrated (0.014%).
Sulfate: Take 0.5g of this product, check according to law (Appendix VIII B of Part II of the Chinese Pharmacopoeia 2000 Edition), and compare with the control solution made of standard potassium sulfate solution 1.5ml, not more concentrated (0.029%).
Heavy metals: Take 1.0g of this product, containing heavy metals must not exceed 20 parts per million.
Arsenic salt: Take 1.0g of this product, should comply with the regulations (0.0002%).
Moisture: Take 0.2g of this product, check according to law (the first law of Appendix VIII M of the second part of the Chinese Pharmacopoeia 2000 edition), not more than 8.0%.
Burning residue: Take 1.0g of this product, the remaining residue should be 18.0% ~ 22.0% (according to anhydrous matter).
Content determination: According to high performance liquid chromatography (Chinese Pharmacopoeia 2000, Part II Appendix V D) determination.
Chromatographic conditions and system suitability: octadecylsilane bonded silica gel was used as filler; Acetonitrile -0.01mol/L phosphoric acid solution (38:62) was the mobile phase; The detection wavelength is 252nm. The theoretical plate number should be no less than 2000 according to the monomonium glycyrrhizinate peak, and the separation degree between monomonium glycyrrhizinate peak and internal standard material peak should meet the requirements.
Preparation of the internal standard solution: Take about 70mg n-butyl p-hydroxybenzoate, weigh it accurately, place it in a 100ml bottle, dissolve it with dilute ethanol and dilute it to the scale, shake well.
Preparation of reference solution: Take about 20mg of monoammonium glycyrrhizate reference product, weigh it accurately, put it in a 100ml bottle, dissolve it with dilute ethanol, add 5ml of internal standard solution precisely, dilute it with dilute ethanol to the scale, and shake well.
Determination method: precisely absorb 5ml of the control solution and 5ml of the test solution, inject them into the liquid chromatograph, record the chromatogram, and calculate the peak area by internal standard method.
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