Pumpkin Seed Protein Powder

Shaanxi Yuantai Biological Technology Co., Ltd. is one of the most reliable manufacturers and suppliers of pumpkin seed protein powder in China. With abundant experience, we warmly welcome you to wholesale bulk pumpkin seed protein powder for sale here from our factory. Quality products and reasonable price are available.

Pumpkin Seed Protein Powder comes from the mature seeds of the Cucurbita plant Cucurbita moschata (Duch.) Poiret. Pumpkin seeds are also called pumpkin kernels, white melon seeds, pumpkin seeds, etc. Pumpkin seeds are rich in nutrients, containing about 50% fat and 30% to 40% protein. After defatting, the protein content of pumpkin seeds can reach more than 60%, and its protein has a rich amino acid composition, making it a good source of plant protein.

It has the characteristics of a high-quality protein source and contains a variety of amino acids needed by the human body, especially essential amino acids such as lysine and threonine. In addition, pumpkin seed protein is also rich in unsaturated fatty acids such as linoleic acid and linolenic acid, as well as minerals such as zinc, magnesium, and iron. It is also rich in antioxidants such as vitamin E and flavonoids.

Due to its rich nutritional content and various benefits, pumpkin seed protein is widely used in health products, nutritional products, food processing and other fields, and is considered a natural nutritional supplement that is beneficial to health.

Main ingredients

The high content of protein and dietary fiber, low content of fat and sodium in pumpkin seed protein makes it the first choice among many plant-based proteins for those who seek to optimize the body and enhance physical fitness. For dieters who need a low-calorie diet, using pumpkin seed protein to replace part of the protein in the meal not only reduces the intake of cholesterol and saturated fat, but also achieves a balanced nutritional intake.
The content of unsaturated fatty acids in pumpkin seeds is relatively high, up to 77.073%. Pumpkin seeds also contain a certain amount of pumpkin seed sprout protein.
Pumpkin seeds are also rich in soybean oleic acid, which can nourish brain cells and remove deposits on the inner walls of blood vessels, thereby improving brain function and cerebral blood circulation, which can prevent the occurrence of atherosclerosis to a certain extent.

Improve cardiovascular disease
High plasma cholesterol levels are a major cause of heart disease. Animal fat in the diet can increase plasma cholesterol levels and is an important factor in arteriosclerosis and heart disease. Pumpkin seed protein does not contain cholesterol and can replace animal protein.
According to the research results of the 1998 Nobel Prize winner in medicine, arginine in protein is the only prerequisite for the synthesis of nitric oxide in the body. Pumpkin seed protein powder contains sufficient arginine. Supplementing pumpkin seed protein can promote the synthesis of nitric oxide, relax blood vessels, lower blood pressure, improve blood supply to the heart and brain, enhance the elasticity of blood vessel walls, and effectively prevent cardiovascular diseases.
Improve body immunity
Pumpkin Seed Protein Powder can promote the synthesis of immunoglobulins and effectively enhance humoral immunity; it can also increase the proliferation of T lymphocytes and enhance cellular immune responses; it can also promote the release of a variety of endocrine hormones, including insulin, growth hormone, prolactin, and antibiotics. Diuretic hormones, and catecholamines, etc., achieve the effect of regulating immunity.
Improve male performance
Pumpkin seed protein can increase sperm motility, improve sperm quality, promote sperm fertilization, and provide kinetic energy for sperm movement. The arginine in pumpkin seed protein is not only involved in sperm formation, but also a basic component of various sperm nuclear proteins. It can also dilate arteries and blood vessels through the nitric oxide pathway. Pfizer of the United States developed a treatment called "Viagra" based on this. Impotence medicines.
Promote muscle synthesis
Pumpkin seed protein is different from ordinary vegetable protein in that it contains a large amount of glutamic acid. Glutamine is first synthesized in the human body from glutamic acid. Experimental studies have proven that glutamine can increase muscle cell volume, stimulate muscle protein synthesis, improve muscle vitality, and increase muscle growth. Currently, whey protein is commonly used as the raw material for muscle-building powder on the market. However, whey protein is an animal-derived protein and is not suitable for vegetarians to eat. Whey protein contains dairy allergens, while pumpkin seed protein is a plant protein and does not contain any allergens. , people prone to allergies can eat it with peace of mind.
anti-oxidation
Enzymatically hydrolyze pumpkin seed protein to obtain an enzymatic hydrolyzate, and the DPPH clearance rate was measured to be 83.47%, so hydrolyzed pumpkin seed protein has antioxidant effects.

Nutritional supplement

It is rich in nutrients such as protein, unsaturated fatty acids, vitamins and minerals. It can be used as a nutritional supplement to meet the body's needs for various nutrients. It is especially suitable for vegetarians and people with insufficient protein intake.

Enhance immunity

Rich in minerals such as zinc, magnesium, and antioxidants such as vitamin E. These ingredients help enhance the body's immune function, improve resistance, and reduce the occurrence of infections and diseases.

Promote muscle growth and repair

It is rich in amino acids, especially essential amino acids such as lysine and threonine, which play an important role in promoting muscle growth and repair, so it is suitable for athletes, bodybuilders and people who need muscle healing.

Improve skin condition

Vitamin E and other antioxidant ingredients in pumpkin seed protein can help protect the skin, delay skin aging, reduce the occurrence of skin problems, and improve skin condition, so it can be used in beauty and skin care products.

Regulate blood lipids and blood sugar

Unsaturated fatty acids such as linoleic acid and linolenic acid in pumpkin seed protein have a certain effect on regulating blood lipids and blood sugar, and help prevent cardiovascular disease, diabetes and other related diseases.

Protect cardiovascular and cerebrovascular health

Rich in magnesium, linolenic acid and other ingredients that help lower blood pressure, improve heart function, and prevent arteriosclerosis and cardiovascular and cerebrovascular diseases.

Reduce menopausal syndrome symptoms

The phytoestrogen-like substances in pumpkin seed protein have a certain effect on women's menopausal syndrome and can help reduce hot flashes, mood swings and other symptoms.

Functional food additive

Due to the high-quality nutrients and good functionality of pumpkin seed protein, it can be used as a high-quality protein additive in food processing to enhance the nutritional value and taste of food.

Pumpkin seeds contain 30% to 40% protein and are rich in amino acids. They are a good plant protein resource. At present, there are relatively few studies on ACE inhibitory peptides derived from pumpkin seed protein, and the possible ACE inhibitory molecular mechanism of pumpkin seed peptides is not clear enough. Therefore, alkaline protease was used to hydrolyze pumpkin seed protein to release ACE inhibitory peptide. The ACE inhibitory peptide was obtained through membrane separation technology and its structure was identified through mass spectrometry technology. At the same time, ACE inhibitory activity experiment, spontaneous hypertensive rats (SHRs) model, and inhibition were used. Kinetics and molecular docking methods were used to study the antihypertensive activity and enzyme inhibition mechanism of ACE inhibitory peptides, in order to provide a theoretical basis for the development of pumpkin seed antihypertensive peptides.

1. Hydrolysis of pumpkin seed protein and activity analysis of membrane separation components of hydrolyzate

As shown in Figure 1A, the degree of hydrolysis of pumpkin seed protein is positively correlated with the hydrolysis time. The degree of hydrolysis increases rapidly within 1 hour after the start of hydrolysis, reaching 7.99%; the degree of hydrolysis increases by 2.99% within 60 to 200 min, and the hydrolysis reaction rate It slowed down significantly, and the degree of hydrolysis reached 11.23% after 4 hours. The pumpkin seed protein fully released its active peptides under the action of alkaline protease.

As can be seen from Figure 1B, at a mass concentration of 1 mg/mL, the PPH ACE inhibitory activity also varies depending on the molecular weight of the hydrolyzate. The ACE inhibitory activity of PPH and peptide components less than 1 kDa is higher, and there is no significant difference in ACE inhibitory activity between the two (P>0.05), which are 46.81% and 46.22% respectively; except for the 3-5 kDa component, the ACE inhibitory activity The activity gradually decreased as the molecular weight of PPH increased, and the ACE inhibitory activity of the 5-10 kDa component was the lowest, with an inhibition rate of 33.74%.

The antihypertensive activity of PPH is shown in Figure 1C. After intragastric administration of SHRs with PPH (100 mg/kg mb), PPH and PPH components less than 1 kDa showed similar antihypertensive effects at 4 h, and both decreased. The systolic blood pressure of SHRs was about 14.80 mm Hg, and the PPH component lower than 1 kDa showed a blood pressure lowering effect when administered for 6 hours, reducing the systolic blood pressure of SHRs by about 21.42 mm Hg. The PPH component lower than 1 kDa showed a blood pressure lowering effect. The activity is better than that of PPH; although the PPH component below 1 kDa shows sustained blood pressure lowering effect, it is still worse than the blood pressure lowering drug captopril.

2. Identification of pumpkin seed protein peptide structure

In order to determine the structure of the peptides that may cause the activity, HPLC/Q-TOF-MS/MS was used to identify the pumpkin seed protein peptide composition in the PPH fraction below 1 kDa. On the basis of obtaining the total ion chromatogram and primary mass spectrum (Figure 2) of the PPH component below 1 kDa, PEAKS Studio software was used to analyze and compare the UniProt database to identify the PPH component below 1 kDa. 9 kinds of polypeptides were found, namely Leu-Leu-Val (LLV), Leu-Val-Phe (LVF), Leu-Thr-Pro-Leu (LTPL), Ser-Val-Leu-Phe (SVLF), Leu-Leu -Pro-Gln (LLPQ), Met-Leu-Pro-Leu (MLPL), Leu-Leu-Pro-Gly-Phe (LLPGF), Val-Leu-Leu-Pro-Glu (VLLPE), Arg-Phe-Pro -Leu-Leu (RFPLL). In addition, by trying to search the bioactive peptide database, it was found that none of the 9 pumpkin seed peptides identified appeared in these ACE inhibitory peptides or antihypertensive peptide data. Only similar antihypertensive peptide sequences were found, such as LLY, LVY, LVE, LLP, LLF, etc., indicating that this study discovered new ACE inhibitory peptides from PPH.

3. ACE inhibitory activity and blood pressure lowering activity of pumpkin seed peptide

As shown in Figure 3A, the ACE half inhibitory concentrations (IC50) of nine peptides RFPLL, VLLPE, LLPGF, MLPL, LLPQ, SVLF, LTPL, LVF, and LLV are 0.94, 16.57, 0.48, 0.36, 5.32, 7.11, 5.07, respectively. 0.17, 3.16 mmol/L, among which the IC50 of LLPGF, MLPL, LVF and RFPLL are all lower than 1 mmol/L, indicating high ACE inhibitory activity.

The antihypertensive activity of pumpkin seed peptides LLPGF, MLPL, LVF and RFPLL, which have better ACE inhibitory activity, was further evaluated (Figure 3B, C). Overall, RFPLL has good blood pressure lowering activity. After 6 hours of intragastric administration of SHRs, the systolic blood pressure is reduced by 37.0 mm Hg and the diastolic blood pressure is reduced by 17.0 mm Hg. The second is LVF. After 6 hours of intragastric administration of SHRs, the systolic blood pressure is reduced. 22.2 mm Hg, diastolic blood pressure decreased by 11.2 mm Hg; while LLPGF and MLPL have relatively poor antihypertensive activity; RFPLL and LVF still have certain antihypertensive activity after administrating SHRs for 24 hours, and at the tested doses, RFPLL and Cato The blood pressure lowering effect of Pullin is relatively similar, indicating that better blood pressure lowering peptides are obtained from pumpkin seed protein.

4. Kinetic analysis of pumpkin seed peptide inhibiting ACE

As can be seen from Figure 4, RFPLL is a mixed inhibitor of ACE. At low concentrations (0.5 mmol/L), RFPLL competitively inhibits ACE, and at higher concentrations (1 mmol/L), it shows a non-competitive inhibitory effect; MLPL's dual The reciprocal straight line approximately intersects with the y-axis, indicating that its inhibition mode of ACE may be competitive inhibition, while LLPGF and LVF may be non-competitive inhibition types.

5. Molecular docking of pumpkin seed peptide and ACE
The results show that RFPLL, LLPGF, MLPL and LVF can all dock well with the active center residues of ACE. Among them, RFPLL forms three hydrogen bonds with the residues Tyr523, Glu162 and Asp377 of ACE, and connects them through O31 and O28. Chelating Zn2+, a lower electrostatic potential energy of -231.64 kJ/mol was obtained, indicating that RFPLL can exert inhibitory activity by binding to the ACE active sites S1, S1' and the triplet catalytic site; the small molecule peptide LVF and the five residues of ACE Nine hydrogen bonds are formed between His353, Ala354, Tyr520, Lys511, and Gln281, and the distance between O2 and Zn2+ is only 2.78 Å.

Food industry: It can be used as a high-quality plant protein additive to increase the protein content of food. It can be used in various food processing such as bread, biscuits, meat products, nutrition bars, cereal foods, condiments, etc. to improve the nutritional value and taste of the products, while also meeting the needs of vegetarians and people with insufficient protein needs.

Nutritional and health care products: Rich in a variety of nutrients, such as protein, unsaturated fatty acids, vitamins and minerals, etc., they can be used to produce nutritional and health care products, such as protein powder, protein drinks, nutritional supplements, etc. These products can meet the body's needs for various nutrients and are especially suitable for vegetarians, athletes and people who need additional nutritional support.

Pharmaceutical field: It has antioxidant, anti-inflammatory, immunomodulatory and other functions, so it can be used in the pharmaceutical field to prepare health foods and drugs. The ingredients of pumpkin seed protein can help enhance immunity, improve muscle healing, regulate blood lipids and blood sugar, and protect cardiovascular and cerebrovascular health. Therefore, it has broad application prospects in the development of health products and drugs.

Beauty and skin care products: The vitamin E and other antioxidant ingredients in pumpkin seed protein are beneficial to skin protection and repair, and can be used to produce skin care products, facial masks, lotions and other beauty products. These products can help improve skin condition and delay skin aging, and are favored by consumers.

Animal feed: Pumpkin Seed Protein Powder can also be used as high-quality animal protein feed for livestock and poultry breeding. It can increase the protein content of feed, improve the nutritional structure of feed, promote animal growth and development, and improve the production performance of livestock and poultry.

YTBIO Pumpkin Seed Protein is made from high-quality, non-GMO pumpkin seeds. It is produced through low-temperature cold-pressing defatting, fine grinding, and targeted separation processes, making it a highly nutritious and hypoallergenic plant protein source.

Naturally Hypoallergenic: Pumpkin seed protein does not contain common allergens.

Amino Acid Ratio: Rich in arginine, glutamic acid, and branched-chain amino acids.

Rich in Minerals: Naturally contains trace elements such as magnesium, zinc, and iron.

Mild Flavor: Pumpkin seed protein has a light nutty aroma and is easily blended with other ingredients.

Clean Label: Physical extraction process, no chemical solvent residue, compliant with natural, non-GMO, and additive-free standards.

If you are interested in YTBIO pumpkin seed protein, please contact us for samples and technical support.

Telephone：+8617782577059
Email：sales@sxytbio.com

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