Nattokinase Powder

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About The Detailed Introduction Of Nattokinase?

Nattokinase powder (NK) (EC 3.4.21.62) is an alkaline serine protease secreted by Bacillus subtilis (natto). It has the advantages of long half-life, strong specificity, small side effects, and can be taken directly orally.
Extracted from food natto or produced by natto bacteria, it is a protein with a molecular weight much smaller than UK, SK, and tPA, and can be absorbed by the intestine. The thrombolytic properties of nattokinase in vitro and in vivo have also been determined through experiments. At the same time, it was concluded that the thrombolytic activity of nattokinase in vivo is four times that of fibrinolysis. It acts quickly and lasts long in the body. It can also activate tPA in the body to gently and continuously increase the fibrinolytic activity of the blood. Patients at risk of myocardial infarction need to take 300,000 units of urokinase at a time, and 50 grams of natto contains 800,000 units of urokinase for its thrombolytic effect. (See the report by Matheson and others that each gram of wet weight of natto is approximately equivalent to 1,600 urokinase units.)
The Research Center for Treatment of Difficult Diseases at St. Mariana Medical University in Japan and the Japan Institute of Biological Sciences recently discovered that nattokinase has the effect of inhibiting platelet coagulation and helps prevent hypertension and arteriosclerosis.
According to the Nikkei Business News, researchers asked subjects to consume a cultured extract of nattokinase and then tested their blood. They found that 6 hours after consuming the extract, the coagulation of the test subjects' platelets was inhibited. Researchers concluded that nattokinase has the function of dissolving blood clots and smoothing blood flow.
Therefore, nattokinase has become a new type of oral thrombolytic drug (second-generation thrombolytic drug), which plays an active role in preventing and treating cardiovascular and cerebrovascular embolism and embolic Alzheimer's disease.

Product Details List

Physical and chemical properties

1.Stability of Nattokinase
pH stability is relatively stable under the natural pH value. When the pH value rises from 7 to 12, it is stable within 10 minutes at room temperature. When the pH value is lower than 5, it is extremely unstable and loses activity quickly. When the temperature exceeds At 60, it rapidly becomes inactive due to protein denaturation. After five cycles of repeated freezing and thawing, more than 95% of its activity was still preserved, indicating that freezing and thawing had no major impact on the activity of nattokinase. When nattokinase is mixed with boiled wheat extract, boiled broth, serum albumin and gastric mucus protein, the stability of nattokinase is significantly increased, and the enzyme activity is not completely lost even at acidic pH. Thermal stability: When the temperature is lower than 45°C, the activity is relatively stable. When the temperature is higher than 60°C, it is rapidly inactivated due to protein denaturation. Freezing and thawing have no major impact on the activity of nattokinase. After repeated freezing and thawing 5 times, the activity of nattokinase is relatively stable. The enzyme can still maintain more than 95% activity. Metal ion stability The activity of nattokinase is also affected by some metal ions. Hg2+ will completely inactivate it. Cu2+, Zn2+, Al3+, Ca2+ and other ions have different inhibitory effects on the enzyme, while Mg2+ and Co2+ have different inhibitory effects on it. activation effect. The stability of other substances, E-ACA and EDTA, have no significant effect on the activity of nattokinase, while PMSF can completely inhibit its activity. 1mmol/L DFP and neguvon can completely inhibit the activity of nattokinase.
2.molecular weight
Nattokinase is a single-chain polypeptidase. Due to different technical methods for measuring molecular weight, the measured molecular weight is also significantly different. Using the SephadexG100 column gel filtration method, the result obtained was 20,000, and the result measured by the circular dichroic method was 27,300. The exact molecular weight of the enzyme was calculated based on the amino acid sequence to be 27.728.
3.PI value (isoelectric point)
Using Svunsson's column electrophoresis method and isoelectric focusing, nattokinase has a symmetrical single plasmin peak, and its PI value is 8.6±0.3. The isoelectric point can be provided. When isolating and purifying nattokinase, consider adjusting the pH value near the PI value, which is beneficial to the purification of the enzyme.
4.Substrate specificity of nattokinase
As a serine protease, NK's amidolytic activity can be observed with many synthetic substrates. The most sensitive substrate to nattokinase is the plasma plasmin substrate, namely S2251; it has certain activity on the substrates H2DLArgPNA, S2238, S2266 and S2302, but is obviously inactive towards S2444 and S2484. Nattokinase has its specific amino acid action and recognition site for hydrolyzing proteins.

Physical and chemical properties

A. It rapidly denatures and becomes inactive when the temperature exceeds 50°C, but repeated freezing and thawing has little effect on it.

B. When the pH value rises from 7 to 12, the activity is stable; when the pH value is lower than 5, it rapidly denatures and becomes inactive. The pH value in the gastric acid environment is only between 1.2 and 2, so it is difficult for nattokinase to survive.

C. It is a macromolecular single-chain polypeptidase that can be decomposed into amino acid fragments or peptide chains with smaller molecular weight by intestinal digestive juices (chymotrypsin, trypsin, small intestinal juice, etc.).

What Are The Benefits?

Research shows that nattokinase has fibrinolytic activity and can treat and prevent thrombotic diseases. At present, the research and application of Natto bacteria mainly focus on the role of nattokinase. However, as a health food, natto also has a variety of health functions, such as thrombolysis, blood pressure lowering, antibacterial effects, etc. It can prevent osteoporosis, improve protein digestibility, antioxidant, etc.
1. Thrombolytic effect
In 1987, Sumi Yoko and others from Tomisaki Medical University in Japan first discovered that the extracted nattokinase was then used to treat thrombosis in dogs and proved to be an enzyme preparation with fibrinolytic activity, so it was named nattokinase.
A study of 200 substances with oral fibrinolytic effects found that nattokinase is the most potential fibrinolytic protease discovered so far. Compared with urokinase and streptokinase, it has a long half-life in the body, can gently and continuously increase the fibrinolytic activity of blood, and is less likely to cause bleeding.
2. Thrombolytic mechanism
① Make the plasma pressure D-dimer positive, directly decompose thrombus, and significantly increase the content of fibrin degradation product FDP;
② Activate plasminogen activator, stimulate vascular endothelial cells to produce tissue plasminogen activator, dissolve fibrin, degrade thrombus, and activate urokinase in the body into urokinase. Urokinase and t-PA activate fibrin together. Lysinogen, dissolves blood clots. It can also inactivate the plasminogen activator blocker PAI-1, further activate more urokinase and t-PA, and promote the dissolution of blood fibrinoprotein. And break down the thrombus;
③Inhibit the endogenous and exogenous coagulation system to achieve anti-thrombolytic and thrombolytic effects. Mainly by increasing TT, it significantly reduces plasma ELT and affects the body's coagulation factor, namely Fig, and significantly prolongs APTT, CT and PT;
④ This enzyme is a serine protease that can directly hydrolyze cross-linked fibrin, but is not sensitive to fibrinogen. Therefore, when the enzyme exerts its fibrinolytic effect, it does not hydrolyze plasma fibrinogen and is unlikely to cause bleeding.
3. Advantages of thrombolysis
As a thrombolytic drug, it has the following advantages: Nattobacterium has been certified by the FDA as a safe bacterium in the food industry; Nattokinase has a small molecular weight and is easier to be absorbed by the human body; it can be absorbed by the digestive tract, so it can become the first oral soluble drug. Thrombus drug; it can directly act on fibrin and act quickly; it can be produced by bacterial fermentation, so it is low-cost and is expected to be developed into a safe edible thromboprophylactic agent.
4. Other pharmacological effects
Nattokinase contains angiotensin-converting enzyme inhibitors that can treat hypertension, so its application in the treatment of hypertension can be further studied. Studies have shown that oral administration can significantly reduce the occurrence of deep vein thrombosis and limb edema. It also plays a role in the prevention and treatment of osteoporosis and diabetes.

Anticoagulant effect: It has the effect of stably reducing platelet aggregation, inhibiting the formation of emboli, and promoting fibrinolysis, thereby preventing the formation and occurrence of thrombosis. These effects make nattokinase a natural anticoagulant.

Blood pressure lowering effect: It can lower blood pressure by reducing platelet activity and improving vascular endothelial function. According to research, long-term use of nattokinase can significantly reduce systolic and diastolic blood pressure in patients with hypertension.

Improves cardiovascular health: Can prevent and treat cardiovascular disease and improve cardiovascular health. It improves endothelial cell function, promotes blood circulation and lowers cholesterol levels, thereby reducing the risk of heart disease, stroke and vascular disease.

Anti-inflammatory effect: Can inhibit inflammatory response and reduce insulin resistance. It can help control inflammatory diseases such as arthritis, asthma and rheumatism, and reduce the occurrence of obesity, metabolic syndrome and other diseases.

Increase fibrinolytic activity: It makes an important contribution to increasing fibrinolytic activity. It can promote the release and production of plasmin, accelerate fibrinolysis, help treat blood clots and prevent symptoms such as cardiovascular disease. Nattokinase can also stabilize the redox potential of human tissues and help maintain human health.

Antibacterial effect: It can enhance human immunity and have direct or indirect antibacterial effect. It can promote the proliferation and secretion of lymphocytes and macrophages, thereby enhancing human immune function. It can also effectively inhibit various pathogens such as Staphylococcus aureus and Streptococcus pneumoniae.

Antioxidant effect: It is rich in flavonoids and has strong antioxidant effect. It scavenges free radicals and other harmful substances, reducing the body's exposure to factors such as environmental pollution and daily stress.

What Are The Application Directions?

Health food field: Nattokinase powder is often used to make health food and health products. It can be used as a nutritional supplement that provides rich nutrients and active enzymes to help enhance the body's immunity, promote digestion and absorption, and improve overall health.

Digestive Health: It is widely used to promote intestinal health. It can improve the balance of intestinal flora, promote the digestion and absorption of food, and reduce gastrointestinal discomfort symptoms such as bloating, constipation, etc.

Blood circulation and cardiovascular health care: Nattokinase powder has anticoagulant and fibrinolytic activities, which can improve blood viscosity, prevent thrombosis, and promote blood circulation. It is widely used to prevent cardiovascular and cerebrovascular diseases and helps reduce cardiovascular risk factors such as high blood pressure and high cholesterol.

Cardiovascular field: Nattokinase is widely used in the prevention and treatment of cardiovascular and cerebrovascular diseases. It can reduce blood viscosity, prevent thrombosis, improve hemodynamics, and reduce the occurrence of thrombotic events. Nattokinase can also help lower blood pressure and improve cardiovascular health.

Anti-thrombotic field: Nattokinase, as a natural anticoagulant, is widely used to prevent the formation of thrombosis. It can promote fibrinolysis, reduce platelet aggregation, prevent the occurrence and further expansion of thrombus, thereby reducing the risk of deep vein thrombosis, pulmonary embolism and other thrombosis-related diseases.

In the field of immune regulation: Nattokinase can enhance the body's immunity, promote the activity of lymphocytes and macrophages, and increase the production of antibodies. It can be used to improve immune function, prevent infectious diseases, and assist in the treatment of immune system-related diseases.

Company introduction and certificates obtained

Shaanxi Yuantai Biological Technology Co., Ltd (YTBIO) was established in 2014, mainly supplying herbal extracts, functional cosmetic raw materials, organic products, etc. We have our own factories, R&D and quality inspection teams, and have the conditions for development, production, and quality assurance. At present, we have obtained ISO9001, ISO22000, HALAL, KOSHER, HACCP, FDA, and other certificates. Therefore, the quality of our products is guaranteed, and it is our original intention to bring high-quality products to customers.

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